The use of biochar has been shown to affect the transport of pathogenic microorganisms. A transport experiment was conducted to determine the effect of biochar application in soil contaminated with amoeba in relation to its transport in sandy soil and to evaluate the effect of coco char and poultry litter char to the attenuation of amoeba.Poultry litter char and charred coconut shells were used as two different sources of biochar and were incorporated at 10 % (w/w) soil-biochar mixture in a polyvinyl chloride (PVC) column (15 cm height and 2.5 cm diameter). There were three treatments and three replicates. One treatment is applied with coco char and the other was with poultry litter char. Control treatment was with no biochar application. Two hundred fifty amoebas were used in the transport experiment and were leached for 15 minutes. Results showed that 30 % of the total amoeba was leached in the saturated column without biochar compared to the less 8-14 % of the total amoeba in the biochar amended columns. Coco char is more effective in attenuating amoeba with 6% advantage over the poultry litter char. Spatial distribution of amoeba in the soil column showed that the number of amoeba accumulated in shallower depths (2-6 cm) in biochar amended columns compared to columns without biochar. Amoeba in coco char amended column accumulated in 2-4 cm only compared to poultry litter char at 4-6 cm. It is shown that incorporation of biochar effectively attenuated the transport of amoeba in a saturated sandy soil. This is where coco char is superior to poultry litter char in terms of attenuation capacity.
keywords: attenuation, amoeba, sandy soil, coco char, poultry char